Composite

Part:BBa_K2637043:Design

Designed by: Jinyu Yang   Group: iGEM18_Tianjin   (2018-10-05)


Gal1 promoter+EYFP+ADH1 terminator+Gal2 promoter+NanoLuc+CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1125
    Illegal SapI site found at 2121


Design Notes

No.


Source

We copied the Gal2 promoter, CYC1 terminator and ADH1T terminator sequences from plasmid BY4741 and utilized the part numbered BBa_E2030 to obtain the sequence of EYFP and the part numbered BBa_K1680009 to gain the sequence of NanoLuc. As for the sequence of Gal1, it is the work from TU Munich's part.

References